Figure 5.

HOS1 Is Required for Nucleo-Cytoplasmic mRNA Export.

(A) In situ hybridization with a fluorescein-labeled oligo(dT) probe in wild-type, hos1, los4, ztl (long period), and toc1 prr9 prr7 prr5 (t975) quadruple mutant (arrhythmic) roots. Plants were fixed at dawn (unless otherwise indicated) after 2 weeks of growth under 12L:12D white-light cycles. Bars = 50 µm.

(B) In situ hybridization with a fluorescein-labeled oligo(dT) in wild-type, hos1, and sar1 roots counterstained with DAPI. Bars = 10 µm.