TABLE 1.

Results by sample^a

Virus	ENIVD sample	Strainb	Copies/ml expected	Copies/ml determinedc	% Of laboratories achieving positive resultsd
Ebola	F6	Mayinga	—e	2.2E7	92.9
	F9	Mayinga	2.2E6	2.7E6	85.7
	F4	Mayinga	2.2E5	1.6E5	78.6
Marburg	F5	Musoke	—	3.6E7	78.6
	F3	Ratayczak	—	3.9E4	64.3
	F10	Ratayczak	3.9E3	3.9E3	57.1
	F18	Ratayczak	3.9E2	<2,647f	35.7
Lassa	L11	AV	4.0E5	5.4E5	85.7
	L4	AV	4.0E4	2.6E4	78.6
	L8	AV	4.0E3	2.6E3	50
	L17	AV	4.0E2	<2,445f	21.4
	L6	Josiah	4.8E6	3.8E6	71.4
	L9	Josiah	4.8E5	7.0E5	85.7
	L3	Josiah	4.8E4	1.2E5	71.4
	L2	Josiah	4.8E3	7.2E3	50
Orthopox	P12	MPX Lam87	5.0E7	3.2E7	100
	P1	MPX Lam87	5.0E6	3.4E6	95.6
	P10	MPX Lam87	5.0E5	5.6E5	81.8
	P5	MPX Lam87	5.0E4	8.0E4	68.2
	P14	MPX Lam87	5.0E3	8.0E3	59.1
	P3	MPX Lam87	5.0E2	1.6E3	45.5
	P6	MPX Lam87	5.0E1	<1,000f	27.3
	P7	CML	8.8E6	8.8E6	95.6
	P9	CPXV 81/02	—	6.0E5	86.4
	P15	CPX Brithon	—	2.4E6	95.6
	P13	VAC	—	1.0E6	90.9
	P11	VAC mod.g	—	1.6E5	86.4
	P4	ECT	—	3.6E6	72.7

^aAll samples available on request through ENIVD (www.enivd.de).

^bOrthopox virus species: monkeypox virus (MPX), vaccinia virus (VAC), cowpox virus (CPX), camelpox virus (CML), mousepox virus (ectromelia virus [ECT]).

^cMeasured by real-time PCR to exclude degradation of virus in plasma after heating and irradiation.

^dCoefficients of correlation of detection rates with virus concentration in samples: filovirus panel, r = 0.914; Lassa virus panel, r = 0.85; and orthopox virus panel, r = 0.914 (P values for the correlations were 0.004, 0.0081, and ≤0.00009, respectively). Positive test results for the total panel for Ebola and Marburg virus, Lassa virus, and orthopox virus, 70, 73, and 78%, respectively; positive test results for samples with ≤10⁴ virus copies, 46, 40, and 44%, respectively.

^e—, virus concentration originally determined in this sample.

^fVirus detected but below linear quantification range of assay.

^gmod., modified.