Figure 3.

Genetic Alteration of CaM KMT Expression Alters H versus N Epidermal Cell Fate determination in Arabidopsis roots.

(A) Single-frame micrographs of roots of wild-type (Col-0), T-DNA insertion KO into CaM KMT mutant (KO), and CaM KMT overexpression line (OX-7) with false color cyan showing H cells and magenta showing N cells. Ectopic N cells (asterisks) form in an otherwise H cell file and ectopic H cells (yellow arrowheads) form in an otherwise N cell file in KO and OX-7 lines. Bar = 100 μm.

(B) Statistical assessment demonstrates a decrease in H cells in KO and OX-7 lines when compared with Col-0. An increase in ectopic N cells is observed in KO and OX-7 lines (n = 40 seedlings for each genotype). After vernalization and germination, individual Arabidopsis seedlings were grown on sterile one-half-strength MS-agar medium vertically for 5 days in light (24 h). Asterisks represent significant difference from the mean at P < 0.05 using the Mann–Whitney U test.