Figure 5.

The CaM KMT KO Mutant Shows Increased Resistance to ABA Stress during Early Seedling Growth.

(A) to (E) Cotyledon greening percentage of Col-0 (squares), CaM KMT KO mutant, and OX-7 seedlings under indicated concentrations of ABA. Seeds were placed on AGM without (A) and with 0.25 (B), 0.5 (C), 0.75 (D), and 1 µM (E) ABA. After stratification at 4°C for 2 d, the plates were transferred to a growth chamber (16-h-light/8-h-dark cycle at 22°C), and cotyledon greening was measured. For the phenotypic measurement, the seedlings having green cotyledons were scored each day from days 2 through 7 after stratification. All assays were repeated three times using ∼50 seeds for each experiment. Data represent mean ± sd (n = 3).

(F) Phenotypic differences observed among Col-0, KO, and OX-7 lines grown without (untreated) or with the indicated concentrations of ABA. The seedlings were digitally imaged under a microscope 3 d after stratification. Bar = 1 mm.

(G) Plant development of seedlings of Col-0, KO, and OX-7 lines under ABA stress. After stratification, seeds were grown in AGM without (untreated) or with 1 µM ABA, grown for additional 7 d, and then photographed.