Figure 2. BAX knockout does not affect sensitivity to 17-AAG in HCT116 human colon cancer cells as measured by SRB or MTT assays.

Exponentially growing HCT116 BAX ^+/− and BAX ^−/− cells were exposed to increasing concentrations of 17-AAG for 96 hours and inhibition of cell proliferation was estimated using the (A) SRB assay or (B) MTT assay. Concentration-response curves were plotted and the GI₅₀ value determined as the compound concentration required to inhibit cell proliferation by 50% compared to controls treated with drug vehicle. N=3, error bars indicate the mean ± SEM.