Figure 7. Del-1 reciprocally regulates p53 expression in mouse primary endothelial cells.

(A) Levels of p53 protein was analyzed by western blotting in primary endothelial cells from Del-1^+/+ and Del-1^−/− mice. Values are means ± SD (n = 3 mice/group). (B) Transcript levels of p53 were analyzed by real-time RT-PCR. WT primary endothelial cells were incubated in the absence or presence of recombinant Del-1 protein (10 nM) for 24 h. Values are means ± SD (n = 4−5 mice/group). *, p < 0.05.