Figure 5. Expression of disorganization genes and activation of the NF-κB pathway are associated with aggressive 3D phenotypes of multiple breast cancer cell lines.

A) Unsupervised hierarchical clustering of breast cancer cell lines in 3D culture using the 180 disorganization genes. Each row represents a gene; each column represents a cell line. B) Bar graph of RelB mRNA levels in basal and luminal types of breast cancer cell lines. RelB mRNA expression is assessed by Affymetrix microarray and measured as log2 (probe intensities). C) Phase and immunofluorescence images of BT549 cells. Cells were either treated with Wedelolactone in 3D culture for 4 days or infected with RelB shRNA retrovirus before being seeded in 3D culture. D) Transwell invasion analysis of BT549 cells. The cells were treated with NF-κB inhibitor (Wedelolactone, 10 μM) or transfected with RelB shRNA vector.