Figure 1. SOCE in hPEC.

A) Average intracellular Ca²⁺ responses (±SEM) from a Fura-2 based Ca²⁺ imaging assay when 100 nM DHT, (n = 44) or no stimulus (n = 14) were applied are blotted vs. time. Extracellular Ca²⁺ concentration is indicated in mM. B) Same as A but either 100 μM ATP, n = 37 (black), or 1 μM Melatonin, n = 14 (green) were used as stimulus. C) qRT-PCR analyses of Orai1, Orai2, Orai3, STIM1 and STIM2 expression levels from hPEC from 17 different patients normalized to TATA box binding protein (TBP) expression as reference gene. D) Ratio of Orai1:Orai3 and STIM1:Orai1 expression levels. E) DHT induced intracellular Ca²⁺ responses in cells transfected with control RNA (black, n = 38), Orai1 siRNA (red, n = 33) or Orai3 siRNA (blue, n = 36). F) Average Ca²⁺ influx rates for cells in E when 2 mM Ca²⁺ and 100 nM DHT were applied. G) Average of Ca²⁺ peaks for cells in E when 2 mM Ca²⁺ and 100 nM DHT were applied when baseline for every cell was subtracted. H) Average Ca²⁺ plateaus for cells in E when 2 mM Ca²⁺ and 100 nM DHT were applied at t = 1600 s and baseline for every cell was subtracted. I) Average Ca²⁺ peaks after store depletion with 100 nM DHT in Ca²⁺ free Ringer for cells in E when baseline for every cell was subtracted.