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. 2013 Nov 1;4(11):2135–2143. doi: 10.18632/oncotarget.1465

Figure 2. Screening chimeric RNA candidates.

Figure 2

Initial screening of 32 chimeric RNAs in ESCC cell lines and patients' tissue was performed by RT-PCR using chimera-specific primers. The expression level was graded based on RT-PCR band intensity. The yellow line demarcates cancer from normal. Left side: ESCC cell lines (EC109, HK2, HK3, EC9706, TE1) and cancer tissues. Right side: matched benign tissues and the immortalized normal esophageal epithelial cells (NE1 and NE3). Most of the chimeric RNAs displayed varying expression levels. ASTN2-PAPPA and GOLM1-MAK10, as marked by arrows, were conspicuously expressed at higher levels in cancer.