TABLE 2.

Genes targeted for multiplex PCR and microarray hybridization

Pathogen	Locusa	GenBank accession no. (reference)	Nameb	Sequence	Annealing temp (°C)	Product (bp)
Aeromonas salmonicida	vapA	M64655 (13)	F-A.sal-1	ATTAGCCCGAACGACAACAC	60	177
			R-A.sal-2	GTCGTTGAATTGGCCTTCAC	60
			P-A.sal-vapA	AACTAAGCAGCCGGTACTGGACTTC	65
	A.plas.	X64214 (18)	F-A.sal-3	TCCGTTGGATATGGCTCTTC	60	101
			R-A.sal-4	TTATCGAGGCAGCCAACAAT	60
			P-A.sal-plas	TCGACACAAAATTCAAATTTAACCCC	65
Listonella anguillarum	rpoN	U86585 (33)	F-V.ang-1	CCAGCAAGAGATCCAAGAGG	60	125
			R-V.ang-2	ACACCTCAGCACTGGCTTCT	60
			P-V.ang-rpoN	CGCTGATGTTCATAGCATCAATGAG	65
	fatA	Z12000 (39)	F-V.ang-3	GTCCGCAAGATGGAATGAAT	60	137
			R-V.ang-4	ACTGCTGCCACTTCCTTTGT	60
			P-V.ang-fatA	AGTTCAGCAAACCTTCCCACAATTT	65
Photobacterium damselae subsp. damselae	ureC	U40071	F-P.dam-1	CACCAGGGGTCTGGAATATG	60	127
	R-P.dam-2	GCTCCAGCTTCAATTTGCTC	60
			P-P.dam-ureC	CTGGAAGCCGTTGATGACTTACCTA	65
	dly	L16584 (26)	F-P.dam-3	GCAATTGTTGGTGAACGATG	60	137
			R-P.dam-4	CGTCGCATGAAATGATCTTG	60
			P-P.dam-dly	GTCAATATGGCCCAGATTGTTTT	65
Vibrio parahaemolyticus	gyrB	AF007287 (42)	F-V.par-1	GCTAAGCAGGGTCGTAATCG	60	145
			R-V.par-2	GACCGATACCACAGCCAAGT	60
			P-V.par-gyrB	CGCAAGAAGTTGCAACGCTTATTAC	65
	toxR	L11929 (27)	F-V.par-3	CTTGGATTCCACGCGTTATT	60	147
			R-V.par-4	TGATTTGCGGGTGATTTACA	60
			P-V.par-toxR	ATCTCAGTTCCGTCAGATTGGTGAG	65
Vibrio vulnificus	cyt	M34670 (44)	F-V.vul-1	TTCATTCGAGCGTGAATTTG	60	100
			R-V.vul-2	ATCAAATACCCAGCCACTGC	60
			P-V.vul-cyt	CCAAGAGCTTGGATGCTATTTCACC	66

^aGenetic locus targeted by the described PCR primers and probes: cyt, cytolysine; gyrB, gyrase B; ureC, urease C; dly, phospholipase D; A.plas., A. salmonicida plasmid.

^bF, sequence of the forward primer; R, sequence of the reverse primer; P, oligonucleotide probe.