TABLE 3.
Results of multiplex PCR and microarray hybridization
| Genetic marker | No. of samplesa
|
Sensitivityb (95% CI) | Specificityc (95% CI) | |||
|---|---|---|---|---|---|---|
| Concordant
|
Discordant
|
|||||
| Positive | Negative | Positive | Negative | |||
| vapA | 3 | 72 | 0 | 0 | 1.0 (0.44-1.0) | 1.0 (0.95-1.0) |
| A.sal | 3 | 72 | 0 | 0 | 1.0 (0.44-1.0) | 1.0 (0.95-1.0) |
| rpoN | 21 | 54 | 0 | 0 | 1.0 (0.85-1.0) | 1.0 (0.93-1.0) |
| fatA | 4 | 69 | 0 | 2 | 0.67 (0.3-0.9) | 1.0 (0.95-1.0) |
| ureC | 6 | 59 | 0 | 0 | 1.0 (0.61-1.0) | 1.0 (0.95-1.0) |
| dly | 5 | 69 | 0 | 1 | 0.83 (0.44-0.97) | 1.0 (0.95-1.0) |
| gyrB | 1 | 74 | 0 | 0 | 1.0 (0.21-1.0) | 1.0 (0.95-1.0) |
| toxR | 1 | 74 | 0 | 0 | 1.0 (0.21-1.0) | 1.0 (0.95-1.0) |
| cyt | 4 | 71 | 0 | 0 | 1.0 (0.51-1.0) | 1.0 (0.95-1.0) |
a
Concordant refers to the number of samples that hybridized correctly to their respective probes (positive) or the number of samples that were correctly scored as negative by multiplex PCR (negative). Discordant refers to the number of samples that showed positive, nonspecific hybridization (positive) or false-negative results (negative).
b
Number of concordant positive samples divided by number of concordant positive samples plus number of discordant negative samples.
c
Number of concordant negative samples divided by number of concordant negative samples plus number of discordant positive samples.