Figure 5.

Recombinant proteins of CLO3 and α-DOX1 act cooperatively to catalyze the conversion of α-linolenic acid into 2-HOT. A to D, HPLC patterns of the products of the reaction of α-linolenic acid with α-DOX1 and CLO3 for 0 h (A) and 24 h (B) and with either α-DOX1 (C) or CLO3 (D) for 24 h. α-DOX1 and CLO3 together generate a new peak (P1) from α-linolenic acid. E, MS/MS profiles of the P1 fraction (top) and a standard of 2-HOT (bottom) identifying the product of the P1 fraction as 2-HOT. F, Possible α-dioxygenase pathway for the production of 2-HOT from α-linolenic acid involving the cooperative action of α-DOX1 and CLO3.