Table II. Threading results of putative plant GPCRs split into groups of proteins that do not share discernible homology.

Putative plant GPCRs with GPCR hits are indicated with single asterisks; no GPCR hits are indicated by double asterisks . Each hit has an associated interpretation, such as high, medium, or low, to indicate the expected reliability. Proteins predicted not to be 7TM proteins are underlined, and the expected number of transmembrane helices is given. There were no hits for all of the expressed protein family 1 (At1g77220 and At4g21570), miscellaneous expressed protein family 4 (At1g49470 and At5g19870), Perl1-like family protein (At1g16560 and At5g62130), and the miscellaneous single members from big gene families (At1g71960, At3g01550, At5g23990, and At5g37310 [predicted approximately nine-transmembrane helical]). There were no hits for the following members of miscellaneous single-copy genes: At1g57680, At2g41610, At2g31440, At3g04970 (predicted six-transmembrane helical), and At3g26090 (RGS1). There were no hits for the following members of miscellaneous single members from small gene families: At3g19260, At2g35710 (predicted six-transmembrane helical), At2g16970 (predicted 12-transmembrane helical), At1g15620 (predicted five/six-transmembrane helical), At1g63110, and At4g36850.

TAIR Locus Identifier	I-TASSER	LOMETS	HHpred	FUGUE	Phyre
Miscellaneous single-copy genes
At1g48270 (GCR1)	* (high)	* (high)	* (high)	* (high)	* (high)
At3g59090	**	**	**	* (marginal)	**
At4g20310 (four-transmembrane helical)	**	**	**	* (guess)	**
Miscellaneous single members from small gene families (eight)
At5g27210	**	**	**	* (guess)	**