Figure 1.

Flg22-induced degradation of endogenous FLS2 is ligand, dose, and time dependent. A and B, Time dependency and specificity in Ler seedlings. Ler wild-type and fls2-24 mutant seedlings were elicited in the presence (+) or absence (–) of 10 μm flg22 (A) or with 1 μm of indicated PAMPs (B) for indicated times. C, Dose and ligand dependency in Col-0 leaves. Col-0 wild-type leaf strips were elicited with (+) or without (–) indicated PAMP with indicated concentration for 0 or 60 min. For immunoblot analyses, total protein extracts were probed with αFLS2 (FLS2), αCalnexin (Caln), or αMPK6 (M6) antibodies. Probing with the latter two antibodies served as loading controls. Each experiment was done at least three times with similar results. AF, inactive flg22 (from A. tumefaciens); E, elf26.