Figure 3.

Desensitization of flg22-induced ROS production was dose dependent. A to D, For ROS production, Col-0 leaf disc halves were elicited for their first elicitation (white square) with the indicated concentration of flg22 at 0 min, washed, and reelicited at 60 min (arrow) with 0.1 μm flg22 (black square; n = 24 per treatment). To allow direct comparisons, all shown ROS experiments (A–D) were performed in the same 96-well plate at the same time. Values are mean ± se. This experiment was repeated more than three times with similar results. RLU, relative light units.