Figure 1.

Analysis of glucosinolate content in seeds of β-oxidation mutants. Structures of specific glucosinolates are depicted. A, 3BZO (CAS no. 80667-69-2). B, 4BZO (CAS no. 75331-11-2). C, 4MTB, 4-Methylthiobutyl glucosinolate (CAS no. 21973-56-8), the most abundant nonbenzoylated glucosinolate. D, Total glucosinolates. Values (μmol g⁻¹ seed) are averages of eight individual parent plants for each genotype. Error bars represent sd (*P < 0.05, **P < 0.01, ***P < 0.001, ANOVA). Plants were grown under long-day conditions, except those marked with asterisks after their name, which were grown in short days. The different shading identifies analyses of mutants and the wild types that were grown in separate batches and processed in separate analytical runs. The full data set including other glucosinolates is provided in Supplemental Table S2, and β-oxidation mutant details are provided in Supplemental Table S1.