Figure 4.

Subunit composition of Rubisco in nontransgenic rice. Total soluble proteins of leaf blade and leaf sheath (2.0 µg for leaf blade and 4.0 µg for leaf sheath) at 5.5 leaf stage were separated on a 3% to 12% gradient gel by blue native-PAGE and stained with Coomassie Brilliant Blue (top). Rubisco bands from blue native-PAGE were excised from the gel and further separated by SDS-PAGE. RbcS proteins were detected by immunoblotting with an antiserum raised against OsRbcS1 (bottom). BN, Blue native; 5-LB, fifth leaf blade; 3-LS, third leaf sheath; 4-LS, fourth leaf sheath.