TABLE 3.
Comparison of LD50 of botulinum toxins produced at 30 and 37°C by C. botulinum strain 93-197
| Dilution | No. of mice alive/no. tested after incubation of culture at:
|
|
|---|---|---|
| 30°C | 37°C | |
| 1:1,280 | 0/2 | 0/2 |
| 1:2,560 | 0/2 | 0/2 |
| 1:5,120 | 1/2a | 0/2 |
| 1:10,240 | 2/2 | 0/2 |
| 1:20,480 | 2/2 | 1/2b |
| 1:40,960 | 2/2 | 2/2 |
The LD50 of type F toxin was 1:5,120 at 30°C; at this dilution and temperature, the LD50 of type B toxin was <1.
The LD50 of type B toxin was 1:20,480 at 37°C; at this dilution and temperature, the LD50 of type F toxin was <1.