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Agarose gel electrophoresis of a 500 bp positive band (S) from a suspected tick (group 5 of Shiraz), Primers are (Tams1, Tams4) followed by (Tams3, Tams2) (a nested pcr was applied) and the infection of tick with T. annulata was confirmed. (Marker is 100 ladder marker) (S= sample)
