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. 2013 Nov 1;5(4):1413–1425. doi: 10.3390/cancers5041413

Figure 2.

Figure 2

Evaluation of the gene silencing efficiency and cell viability. Gene silencing efficiencies and cell viability were evaluated, when 0.1 μg of siRNA was transfected into HeLa cells stably expressing luciferase. (A) Gene silencing efficiencies of R8-D-MEND (E3) (closed circles) and Lipofectamine 2000 (open circles) were evaluated at 12, 24, 48 and 72 h after transfection. Significant differences between the R8-D-MEND (E3) and Lipofectamine 2000 were determined by two-tail unpaired student’s t-test (** p < 0.01 * p < 0.05). Data are means ± S.D. (n = 3–4). (B), Cell viabilities of R8-D-MEND (E3) (closed bar) and Lipofectamine 2000 (open bar) were evaluated at 48 h after transfection. No significant differences between the R8-D-MEND (E3) and Lipofectamine 2000 were found when the two-tail unpaired student’s t-test was applied. Data are the means ± S.D. (n = 3–4).