TABLE 2.
Strains tested and multiplex PCR results obtained in the optimization and validation experiments
| Species tested | Subspecies | Serogroup | Serotype | Phage type | No. of isolates tested | PCR results (bp)a
|
Type codeb | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 102 [1] | 204 [2] | 304 [4] | 401 [8] | 502 [16] | 705 [32] | |||||||
| Citrobacter freundii | 1 | 0 | ||||||||||
| Enterobacter cloacae | 1 | 0 | ||||||||||
| Enterococcus faecalis | 1 | 0 | ||||||||||
| Escherichia coli | 4 | 0 | ||||||||||
| Hafnia alvei | 1 | 0 | ||||||||||
| Klebsiella pneumoniae | 1 | 0 | ||||||||||
| Proteus vulgaris | 1 | 0 | ||||||||||
| Pseudomonas aeruginosa | 1 | 0 | ||||||||||
| Shigella boydii | 1 | 0 | ||||||||||
| Salmonella enterica | I | B | Bredeney | 1 | + | 2 | ||||||
| Agona | 1 | + | 2 | |||||||||
| Brandenburg | 1 | + | 2 | |||||||||
| Heidelberg | 1 | + | 2 | |||||||||
| 4,12:b:− | 1 | + | 2 | |||||||||
| C1 | Braenderup | 1 | + | 2 | ||||||||
| Virchow | 2 | + | 2 | |||||||||
| Infantis | 1 | + | 2 | |||||||||
| D | Miami | 2 | + | 2 | ||||||||
| E | Anatum | 1 | + | 2 | ||||||||
| F | 11:1,v:− | 1 | + | 2 | ||||||||
| G | Cubana | 1 | + | 2 | ||||||||
| Salmonella enterica | II | K | 18:z10:z6 | 1 | + | 2 | ||||||
| Y | 48:k:e,n,x,z15 | 1 | + | 2 | ||||||||
| Salmonella enterica | IIIa | X | 47:z4,z23:− | 1 | + | 2 | ||||||
| Y | 48:z4,z23:− | 1 | + | 2 | ||||||||
| Y | 48:−:− | 1 | + | 2 | ||||||||
| Salmonella enterica | IIIb | X | 47:i:z | 1 | + | 2 | ||||||
| Y | 48:r:z | 1 | + | 2 | ||||||||
| 58 | 58:r:z | 1 | + | 2 | ||||||||
| 58:z52:z | 1 | + | 2 | |||||||||
| Z | 50:i:z | 1 | + | 2 | ||||||||
| Salmonella enterica | IV | H | 6,14:z4,z23:− | 2 | + | 2 | ||||||
| I | 16:z4,z32:− | 1 | + | 2 | ||||||||
| Y | 48:z4,z32:− | 1 | + | 2 | ||||||||
| Salmonella enterica | I | B | Abony | 1 | + | + | 3 | |||||
| D | Dublin | 1 | + | + | 3 | |||||||
| IIIb | 60 | 60:r:e,n,x,z15 | 1 | + | + | 3 | ||||||
| Salmonella enterica | I | D | Enteritidis | Several | 34 | + | + | 6 | ||||
| Salmonella enterica | I | B | Typhimurium | Several | 16 | + | + | 10 | ||||
| Salmonella enterica | I | B | Typhimurium | 104 | 15 | + | + | + | 11 | |||
| 12 | 2 | + | + | + | 11 | |||||||
| C2 | Lindenburg | 1 | + | + | + | 11 | ||||||
| Salmonella enterica | I | C2 | Blockley | 1 | + | + | 18 | |||||
| Fayed | 1 | + | + | 18 | ||||||||
| Glostrup | 1 | + | + | 18 | ||||||||
| Goldcoast | 1 | + | + | 18 | ||||||||
| Hadar | 2 | + | + | 18 | ||||||||
| Litchfield | 2 | + | + | 18 | ||||||||
| Manhattan | 1 | + | + | 18 | ||||||||
| Muenchen | 1 | + | + | 18 | ||||||||
| Newport | 1 | + | + | 18 | ||||||||
| Salmonella enterica | I | C2 | Chailey | 1 | + | + | + | 26 | ||||
| Cremieu | 1 | + | + | + | 26 | |||||||
| Duesseldorf | 1 | + | + | + | 26 | |||||||
| Kottbus | 1 | + | + | + | 26 | |||||||
| Hadar | 1 | + | + | + | 26 | |||||||
| Salmonella enterica | I | B | 4,5,12:i:− | U302 | 10 | + | + | + | + | 43 | ||
a
The PCR result, the presence (+) of 102-, 204-, 304-, 401-, 502-, and 705-bp bands, which were assigned numbers 1, 2, 4, 8, 16, and 32 (shown in brackets), respectively, is shown for the strains tested.
b
The type code is obtained by adding all the assigned numbers to the positive amplicons. Each code is specific for a particular band pattern.