TABLE 3.
Comparison of results obtained analyzing human stool samples by multiplex PCR and by culture and serotyping
| Result by culture and serotyping | No. of samples in the following category by Multiplex-PCR
|
||||||
|---|---|---|---|---|---|---|---|
| Inhibitiona | Negative | Type code 2b | Type code 6 | Type code 10 | Type code 11 | Total | |
| Negative | 9 | 81 | 90 | ||||
| Salmonella serotype Enteritidis | 1 | 2c | 1d | 22 | 26 | ||
| Salmonella serotype Typhimurium | 2 | 2 | 4 | ||||
| Total | 10 | 83 | 1 | 22 | 2 | 2 | 120 |
Inhibition of multiplex PCR.
The type code is obtained by adding all the assigned numbers to the positive amplicons. Each code is specific for a particular band pattern.
False-negative data in multiplex PCR.
Mistaken result in multiplex PCR.