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. 2013 Dec 4;5(4):1643–1654. doi: 10.3390/cancers5041643

Figure 3.

Figure 3

BHA blocks NNK-induced occurrence of F4/80+ macrophages in lungs. (a) A/J mice were maintained on normal or BHA diet for 2 weeks prior to NNK injection. Lungs were collected at 6 months after NNK treatment and sectioned and immunostained for F4/80. Representative images are shown (T: tumor area); (b) Quantitative analysis of (a) is shown. The F4/80+ cells were evaluated by counting 10 high power fields (20×) per lung section (1 mm)/2 tissue sections/ mouse. n = 10 mice; (c) A/J mice were maintained on normal or BHA diet 1 week after NNK treatment. Lungs were collected at 6 months after NNK treatment and sectioned and immunostained for F4/80. Representative images are shown (T: tumor area); (d) Quantitative analysis of (c) is shown.