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PI3K/AKT/mTOR signaling and beclin-1 are involved in matrine-induced autophagy. (a) Western blot analysis of pAKT, total AKT, p-mTOR, total mTOR, and beclin-1 in HepG2 and Bel7402 cells. β-actin is shown as a loading control. Data are from three independent experiments; (b) Analysis of relative expression of pAKT, total AKT, p-mTOR, total mTOR, and beclin-1. *p < 0.05 vs. control group.
