Table 3.
Sensitivity of the wild-type Rhizobium leguminosarum bv. trifolii 24.2 and the mutant strains Rt270, MM3, and MM4 to various stressors.
| Strain | Minimal inhibitory concentration * | |||
|---|---|---|---|---|
|
| ||||
| SDS (% w/v) | DOC (% w/v) | Sarcosyl (% w/v) | Ethanol (% v/v) | |
| Rt24.2 (wt) | 0.025 ± 0.005 a | 0.11 ± 0.005 a | 0.05 ± 0.005 a | 5.5 ± 0.25 a |
| Rt270 (pssA) | 0.020 ± 0.005 a | 0.07 ± 0.005 c | 0.06 ± 0.005 a | 3.5 ± 0.25 b |
| MM3 (pssD) | 0.015 ± 0.005 a | 0.09 ± 0.005 b | 0.05 ± 0.005 a | 5.0 ± 0.25 a |
| MM4(pssJIHGF) | 0.015 ± 0.005 a | 0.085 ± 0.005 b | 0.045 ± 0.005 a | 5.0 ± 0.25 a |
*
Given values are averages of three independent experiments with 3 biological repetitions for each strain and treatment;
a,b,c
indicate statistically significant differences (in column) for an individual stress factor tested (p value <0.05; ANOVA, Tuckey’s test).