Table 2.
Sensitivity tests of RT-LAMP, RT-qPCR and conventional RT-PCR methods using PBMC from positive samples.
| Assay | PBMC/1 × 106 BJAB cells | |||||||
|---|---|---|---|---|---|---|---|---|
|
| ||||||||
| 106 | 105 | 104 | 103 | 102 | 101 | 100 | ||
| RT-LAMP | EBNA1 | + | + | + | + | − | − | − |
| EBNA2 | + | + | + | + | ±(2/3) | − | − | |
| LMP1 | + | + | + | + | − | − | − | |
| LMP2A | + | + | + | + | − | − | − | |
| BZLF1 | + | + | + | − | − | − | − | |
|
| ||||||||
| RT-qPCR | EBNA1 | + | + | + | + | − | − | − |
| EBNA2 | + | + | + | + | − | − | − | |
| LMP1 | + | + | + | + | − | − | − | |
| LMP2A | + | + | + | + | − | − | − | |
| BZLF1 | + | + | + | − | − | − | − | |
|
| ||||||||
| Conventional RT-PCR | EBNA1 | + | + | + | − | − | − | − |
| EBNA2 | + | + | − | − | − | − | − | |
| LMP1 | + | + | + | − | − | − | − | |
| LMP2A | + | + | − | − | − | − | − | |
| BZLF1 | + | + | − | − | − | − | − | |
+, triplicate assay showed all positive; ±, triplicate assay showed both positive and negative (positive number/test number); −, triplicate assay showed all negative.