Table 1.
Name | Sequence (5′-3′) | Primer used |
---|---|---|
P9-3F1 | GGTCAAGCAGATCACCGTGaarganttyga | 3′RACE |
P9-3F2 | GGCCTTCCTGTTCTTCTTCgsnggntwyga | 3′RACE |
oligod(T) | AAGCAGTGGTATCAACGCAGAGTAC(T)18VN | 3′RACE |
10 × UPM | Long: CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | 3′RACE |
Short: CTAATACGACTCACTATAGGGC | 3′RACE | |
P9-5R1 | CCGCTCGGGGTCGaayttnthngg | 5′RACE |
P9-5R2 | GGCGCAGGTGGCGatnacrtcrtt | 5′RACE |
Outer Primer | TACCGTCGTTCCACTAGTGATTT | 5′RACE |
Inner Primer | CGCGGATCCTCCACTAGTGATTTCACTATAGG | 5′RACE |
P9ORF-F | ATGGTCTTCATTTACCTCCT | ORF cloning |
P9ORF-R | TCAATTCTTGCGTGGCCTAAAC | ORF cloning |
P9F | GAAAAGTTTACATCCAACCCGAC | Full-length cloning |
P9R | GACGTTATAATATAACGTTTATTTCAG | Full-length cloning |
QCYP9F | AAATACCGCTCTGGGCATAC | qPCR |
QCYP9R | GATACGCCATCACCTTTACTT | qPCR |
QActinF | CGGCATCCACGAAACCACCT | qPCR |
QActinR | TGGAAGGAGCCAGTGCGG | qPCR |