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. 2013 Dec 13;14(12):24211–24229. doi: 10.3390/ijms141224211

Table 1.

PCR primers used in this study.

Name Sequence (5′-3′) Primer used
P9-3F1 GGTCAAGCAGATCACCGTGaarganttyga 3′RACE
P9-3F2 GGCCTTCCTGTTCTTCTTCgsnggntwyga 3′RACE
oligod(T) AAGCAGTGGTATCAACGCAGAGTAC(T)18VN 3′RACE
10 × UPM Long: CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT 3′RACE
Short: CTAATACGACTCACTATAGGGC 3′RACE
P9-5R1 CCGCTCGGGGTCGaayttnthngg 5′RACE
P9-5R2 GGCGCAGGTGGCGatnacrtcrtt 5′RACE
Outer Primer TACCGTCGTTCCACTAGTGATTT 5′RACE
Inner Primer CGCGGATCCTCCACTAGTGATTTCACTATAGG 5′RACE
P9ORF-F ATGGTCTTCATTTACCTCCT ORF cloning
P9ORF-R TCAATTCTTGCGTGGCCTAAAC ORF cloning
P9F GAAAAGTTTACATCCAACCCGAC Full-length cloning
P9R GACGTTATAATATAACGTTTATTTCAG Full-length cloning
QCYP9F AAATACCGCTCTGGGCATAC qPCR
QCYP9R GATACGCCATCACCTTTACTT qPCR
QActinF CGGCATCCACGAAACCACCT qPCR
QActinR TGGAAGGAGCCAGTGCGG qPCR