Table 2.
Candidate reference genes ranked according to their expression stability (M value) as determined by NormFinder.
| Ranking | VS + RS | VS | RS | DS + CS | DS | CS |
|---|---|---|---|---|---|---|
|
| ||||||
| Gene (M value) | Gene (M value) | Gene (M value) | Gene (M value) | Gene (M value) | Gene (M value) | |
| 1 | Actin (0.112) | Actin (0.112) | EF1α (0.020) | Actin (0.139) | Actin (0.116) | Actin (0.133) |
| 2 | EF1α (0.194) | GAPDH (0.185) | UEP (0.110) | GAPDH (0.256) | UBQ-LIKE (0.268) | GAPDH (0.136) |
| 3 | GAPDH (0.230) | TUB8 (0.187) | GAPDH (0.200) | UBQ-LIKE (0.349) | GAPDH (0.309) | EF1α (0.279) |
| 4 | UEP (0.287) | UBQ-LIKE (0.229) | Actin (0.201) | TUB5 (0.419) | TUB5 (0.432) | UBQ-LIKE (0.387) |
| 5 | UBQ-LIKE (0.406) | EF1α (0.289) | TUB8 (0.429) | EF1α (0.434) | TUB8 (0.476) | TUB5 (0.436) |
| 6 | TUB8 (0.424) | TUA3 (0.364) | UEC (0.459) | TUA3 (0.467) | EF1α (0.489) | TUA3 (0.470) |
| 7 | UEC (0.486) | UEP (0.394) | UBQ-LIKE (0.509) | TUB8 (0.497) | TUA3 (0.549) | TUB8 (0.475) |
| 8 | 18S (0.646) | TUB5 (0.416) | TUB5 (0.597) | UEP (0.593) | UEP (0.586) | UEC (0.592) |
| 9 | TUB5 (0.678) | UEC (0.459) | 18S (0.654) | UEC (0.664) | UBQ10 (0.662) | UEP (0.631) |
| 10 | TUA3 (0.886) | 18S (0.692) | TUA3 (0.988) | 18S (0.763) | UEC (0.686) | 18S (0.634) |
| 11 | UBQ10 (1.185) | UBQ10 (0.807) | UBQ10 (1.030) | UBQ10 (0.848) | 18S (0.757) | UBQ10 (0.897) |