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. 2013 Dec 16;14(12):24399–24411. doi: 10.3390/ijms141224399

Figure 1.

Figure 1.

Effects of 5-FU on gastric cancer cell viability and apoptosis. (A) Three gastric cancer cell lines (SGC7901, MGC803, and BGC823) were treated with 5-FU for 48 h. Cell viability was tested by the MTT assay. Then a statistical analysis was performed to compare untreated samples and different concentrations of treated samples. The percentage of untreated samples was 100%; (B) SGC7901, BGC823, and MGC803 were treated with 2 μg/mL 5-FU for 48 h, apoptosis status was observed by flow cytometry, and results were analyzed by Modifit software; (C) MGC803 cells were treated for 48 h with the indicated concentration of 5-FU and mitochondria membrane potential was measured; and (D) MGC803 cells were treated with 2 μg/mL 5-FU for the indicated time. Expression of Bcl-2, Bax and β-actin was detected by western blot. Data are expressed as the mean ± SD. #p < 0.05. Representative results from at least three individual experiments are shown.