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. 2013 Dec 16;14(12):24399–24411. doi: 10.3390/ijms141224399

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 2. — Effects of 5-FU on pEGFR, EGFR, pERK, ERK, pAkt and Akt expression in MGC803 cells. MGC803 cells were treated with 2 μg/mL 5-FU for the indicated time. (A) pEGFR, EGFR, pERK, ERK, pAkt, and Akt expression was evaluated by western blot; and (B) MGC803 cells were treated with 2 μg/mL 5-FU in the absence or presence of MAPK inhibitor of PD98059 or PI3K inhibitors of LY294002. Then the percentage of apoptotic cells was analyzed by flow cytometry. Representative results from at least three individual experiments are shown.

Figure 2. — Effects of 5-FU on pEGFR, EGFR, pERK, ERK, pAkt and Akt expression in MGC803 cells. MGC803 cells were treated with 2 μg/mL 5-FU for the indicated time. (A) pEGFR, EGFR, pERK, ERK, pAkt, and Akt expression was evaluated by western blot; and (B) MGC803 cells were treated with 2 μg/mL 5-FU in the absence or presence of MAPK inhibitor of PD98059 or PI3K inhibitors of LY294002. Then the percentage of apoptotic cells was analyzed by flow cytometry. Representative results from at least three individual experiments are shown.