Figure 3.
Effects of EGFR inhibitor (C225) on pERK, ERK, pAkt and Akt expression upon 5-FU treatmentin MGC803 cells. (A) MGC803 cells were pretreated with 10 μg/mL C225, and then treated with 5-FU for the indicated time. Inhibition rate was tested by MTT assay. The percentage of untreated samples was 100%; and (B) pEGFR, EGFR, pERK, ERK, pAkt, and Akt expression was evaluated by western blot. Representative results from at least three individual experiments are shown.