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. 2013 Dec 16;14(12):24399–24411. doi: 10.3390/ijms141224399

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© 2013 by the authors; licensee MDPI, Basel, Switzerland

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 4. — Effects of Cbl-b on 5-FU chemosensitivity. MGC803 cells were transfected by non-silencing control and Cbl-b shRNA. (A) Cbl-b and β-actinexpression were evaluated by western blot; (B) Then cells were treated with the indicated concentration of 5-FU for 48 h, and cell viability tested by MTT assay. Statistical analysis was performed. The percentage of untreated samples is 100%; (C) MGC803 cells were transfected by non-silencing control and shRNA for Cbl-b, followed by treatment with 2 μg/mL 5-FU for 48 h. Apoptotic cells were observed by flow cytometry, and final results were analyzed with Modifit software; (D) The cells were treated with 2 μg/mL 5-FU for 48 and 72 h and mitochondria membrane potential was measured; and (E) The cells were treated with 2 μg/mL 5-FU for 6 and 48 h. Expression of Bcl-2, Bax and β-actin were detected by western blot. ^#p < 0.05. Representative results from at least three individual experiments are shown.

Figure 4. — Effects of Cbl-b on 5-FU chemosensitivity. MGC803 cells were transfected by non-silencing control and Cbl-b shRNA. (A) Cbl-b and β-actinexpression were evaluated by western blot; (B) Then cells were treated with the indicated concentration of 5-FU for 48 h, and cell viability tested by MTT assay. Statistical analysis was performed. The percentage of untreated samples is 100%; (C) MGC803 cells were transfected by non-silencing control and shRNA for Cbl-b, followed by treatment with 2 μg/mL 5-FU for 48 h. Apoptotic cells were observed by flow cytometry, and final results were analyzed with Modifit software; (D) The cells were treated with 2 μg/mL 5-FU for 48 and 72 h and mitochondria membrane potential was measured; and (E) The cells were treated with 2 μg/mL 5-FU for 6 and 48 h. Expression of Bcl-2, Bax and β-actin were detected by western blot. ^#p < 0.05. Representative results from at least three individual experiments are shown.