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Thin layer chromatography (TLC) of the κ-carrageenase degradation products. Enzymatic hydrolysis of κ-carrageenan was conducted under standard condition with 0.5% κ-carrageenan as substrate and reaction for 5 min (1); 30 min (2); 1 h (3); 2 h (4); 3 h (5); 6 h (6); 9 h (7); 12 h (8); 24 h (9) and 48 h (10), respectively. M, control (galactose). Combined with HPLC and mass spectrometry results, a, b and c were ê-neocarraoctaose, κ-neocarrabiose and κ-neocarrabiose respectively.
