Table 1.
Purification of the κ-carrageenase from Cellulophaga lytica strain N5-2.
| Step | Volume (mL) | Total protein a (mg) | Total activity b (units) | Specific activity (units/mg) | Folds | Recovery (%) |
|---|---|---|---|---|---|---|
| Cell-free medium | 1000 | 56.5 | 1648 | 29 | 1 | 100 |
| 40% and 80% (NH4)2SO4 precipitate | 80 | 11 | 1145 | 104 | 4 | 69.5 |
| SephadexG-200 gel-filtration | 5 | 0.86 | 580 | 675 | 23 | 35.2 |
| SephadexG-75 gel-filtration | 2 | 0.235 | 285 | 1170 | 40 | 17.3 |
a
Protein contents were determined by Bradford method;
b
Carrageenase activity was determined by the reducing power method of 3,5-dinitrosalicylic acid (DNS).