Figure 1.

Metformin inhibits the proliferation of bladder cancer cells. (A) 5637 (a) and T24 (b) cells (5 × 10³ cells/well) were seeded in 96-well culture plates. After 24 h, cells were treated with metformin (0, 2, 5, 10, 20 mM) for another 48 h. Cell viability was measured by MTT assay. The results were expressed as percent of cell viability compared with control (0 mM). Columns, means of three independent experiments; bars, SEs; (B) 5637 (a) and T24 (b) cells (5 × 10⁴ cells/well) were seeded in 12-well culture plates. After treatment as in panel A, cell numbers were determined using a hemocytometer. The results were expressed as percent of viable cells compared with control. Columns, means of three independent experiments; bars, SEs; (C,D) 5637 (a) and T24 (b) cells were treated with metformin (Met) at different concentrations for 24, 48 and 72 h. Cell proliferation was measured by MTT (C) or cell count assay (D). Data, means of three independent experiments; bars, SEs. *p < 0.05 versus control; **p < 0.01 versus control.