Figure 2.

Metformin inhibits colony formation of bladder cancer cells. (A) 5637 and T24 cells grown in 6-well culture plates were treated with the indicated concentrations of metformin, every third days for two weeks. The pictures of 6-well culture plates with colonies were taken by a digital camera on day 14; and (B) The bar graph was obtained by calculating the percentages of colony numbers relative to controls, defined as 100%, measured by 1-D gel quantity software Quantity One. Columns, means of three independent experiments; bars, SEs. *p < 0.05 versus control; **p < 0.01 versus control.