TABLE 2.
cDNA cloning primers
| Fragment | Primers used for cDNA amplification and cloning (sequence) | Cloning vector and cloning sites |
|---|---|---|
| Sa | HTAs+1 (CGCGGATCCGATGTTTATTTTCTTATTATTTCTT) | pFastBac Hta, BamHI, KpnI |
| S-5 (CGGGGTACCTGCAGCTGACGTGCCCCAAATGTC) | ||
| Sb | HTAs+2 (CGCGGATCCGATTTGGGGCACGTCAGCTGCAGCC) | pFastBac Hta, BamHI, KpnI |
| S-4 (CGGGGTACCCCTAAGCTTGCCATGTCTAAGATA) | ||
| Sc | HTAs+3 (CGCGGATCCGTATCTTAGACATGGCAAGCTTAGG) | pFastBac Hta, BamHI, KpnI |
| S-3 (CGGGGTACCAAAGTTAGTAGGTATAGCAAT) | ||
| Sd | HTAs+4 (CGCGGATCCGCATACAGTTTCTTTATTACGTAGT) | pFastBac Hta, BamHI, KpnI |
| S-2 (CGGGGTACCAAGGATATCATTTAGCACACTTGA) | ||
| Se | HTA+5 (CGCGGATCCGTCAAGTGTGCTAAATGATATCCTT) | pFastBac Hta, BamHI, KpnI |
| S-1 (CGGGGTACCTTATGTGTAATGTAATTTGACACC) | ||
| Sc | S+3 (CGCGGATCCTATCTTAGACATGGCAAGCTTAGG) | pQE30, BamHI, KpnI |
| S-3 (CGGGGTACCAAAGTTAGTAGGTATAGCAAT) |