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. 2013 Dec 9;110(52):21153–21158. doi: 10.1073/pnas.1320208110

Fig. 2.

Fig. 2.

Activity of dopamine and not serotonin neurons affects oviposition preference. (A) Dopaminergic neuron cell body positions in one hemisphere of the adult central brain are marked based on anti-TH immunohistochemistry (27, 28, 31, 32). PAM cell number is underrepresented in the schematic (33). (B) Cells colabeled with anti-TH antibody and Ddc-GAL4 in the central brain (33). Disrupting neurotransmission in Ddc cells decreased oviposition preference [n = 17–21 per strain; ANOVA: F(3,73) = 47.51, P < 0.0001]. (C) Schematic of the TH-GAL4 expression pattern in the central brain (33). See Fig. S5 for more details. Arrow highlights that most PAM neurons do not express TH-GAL4. Disrupting neurotransmission in TH cells increased oviposition preference [n = 9–16 per strain; ANOVA: F(3,51) = 9.62, P < 0.0001]. (D) Disruption of transmission in serotonergic neurons with TRH-GAL4 did not disrupt oviposition preference [n = 19–21 per strain; ANOVA: F(2,76) = 4.23, P = 0.08; all Tukey’s comparisons to TRH/TeTx: P > 0.05]. Bars on graphs represent means ± SEM. *P < 0.05; **P < 0.001; ***P < 0.0001. Clusters of dopaminergic neurons are named based on their location in the brain: PAM, protocerebral anterior median; PPL, protocerebral posterior lateral; PPM, protocerebral posterior median; PAL, protocerebral anterior lateral; Sb, subesophageal ganglion.