Fig. 3.

Quantification of resolution enhancement using single Atto 655 molecules on glass. (A) Comparison among conventional spin disk, CSD-ISM, and CSD-ISM with Fourier reweighting of a single Atto 655 molecule. The spin-disk image (D) was taken at an exposure time of 1 s. The CSD-ISM image (E) was calculated from 1,000 single images, each with an exposure time of 138 µs (∼80 s acquisition time). (B) Plot of a section through the center of the molecule for the different images in A. The FWHMs (Gauss fit) of the sections are 248 ± 12 nm, 186 ± 3 nm, and 171 ± 2 nm (SE), respectively. The histogram of FWHMs of single-molecule fluorescence (C) is determined by a 2D Gauss fit. The histogram contains both the x and y values of the FWHM of 86 single-molecule spots. The scale bar is 300 nm in A and 4 μm in D and E.