Fig. 3.

FLT3/D835Y mice develop a less aggressive MPN and less significant block in B-cell development relative to FLT3/ITD mice. (A) Twelve-week-old FLT3/D835Y mice have intermediate spleen size compared with the wild-type and FLT3/ITD mice. (B) Representative FACS analysis and graphical presentation of Mac1 and Gr1 expression in BM from 12-wk-old wild-type, FLT3/D835Y, and FLT3/ITD mice. (C) H&E staining of BM and spleen of all three genotypes at 3 and 12 mo. (Scale bar, 100 μM.) (D) Immunophenotypic analysis using B-lymphocytic-related markers of the BM of 12-wk-old mice. (E) Graphical presentation of the frequencies of cells at different stages of B-cell development, as assessed in D. Data are representative of 3 separate experiments (n = 3 for each experiment). (F) Expression levels of transcription factors in B220⁺CD43⁺CD19⁻ cells from all of the three types of mice, evaluated by quantitative RT-PCR. Data are expressed as mean ± SEM (error bars); P values on graphs: NS, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.