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. 2013 Dec 31;3:316. doi: 10.3389/fonc.2013.00316

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Copyright © 2013 Huang, Belharazem, Li, Kneitz, Schnabel, Rieker, Körner, Nix, Schalke, Müller-Hermelink, Ott, Rosenwald, Ströbel and Marx.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Several cell lines (1889c, HaCat, TE167, and PC3) were transfected using si-BIRC3 for 48h, which significantly repressed expression of (A) Birc3 mRNA ****p < 0.0001 in 1889c, HaCat and TE167 cells and ***p = 0.0002 in PC3) and (B) Birc3 protein. After transfection. Changes of the cytology of transfected cells is shown in (C). Cells were stained with an antibody to cleaved caspase 3 for apoptosis detection (immunoperoxidase ×20) (D). FACS analysis to quantify apoptosis using Annexin V-FITCS/PI (E). All experiments were done in triplicate and each measurement in duplicate. Similar low levels of apoptosis following si-BIRC3 transfection were observed in A549 (lung carcinoma); MCF7 (breast carcinoma); and HeLa (cervical adenocarcinoma) cells.