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. Author manuscript; available in PMC: 2013 Dec 31.
Published in final edited form as: Cell Host Microbe. 2011 Dec 8;10(6):10.1016/j.chom.2011.10.013. doi: 10.1016/j.chom.2011.10.013

Figure 5. Mutagenesis Analyses of AvrPtoB250–359-BAK1-KD Complex.

Figure 5

(A) Effects of point mutations in BAK1 on the interaction with AvrPtoB. BAK1-KD variant proteins were individually incubated with AvrPtoB250–359 and subjected to gel filtration. Aliquots of the peak fraction corresponding to BAK1-KD were visualized by Coomassie blue staining following SDS-PAGE.

(B) Effects of point mutations in AvrPtoB on the interaction with BAK1. The assay is similar to (A) except that AvrPtoB mutant proteins were used to test interaction with wild-type BAK1-KD (see also Figure S3).