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. Author manuscript; available in PMC: 2013 Dec 31.
Published in final edited form as: J Cell Biochem. 2009 Oct 1;108(2):10.1002/jcb.22263. doi: 10.1002/jcb.22263

Figure 1. CoAA Interacts with Runx2 In Vivo.

Figure 1

A. CoAA Immunoprecipitates with Runx2. Whole cell lysates (WCL) of the rat osteosarcoma cell line, UMR-106-1 were immunoprecipitated with Protein G Dynabeads alone (no IgG), or antibodies to CoAA, Runx2, or FHOD1 (control IgG). Proteins were transferred to membranes and detected by immunoblotting with CoAA antibodies. IgGH denotes the immunoglobulin heavy chain. B. The co-localization (yellow) of Runx2 (red) and CoAA (green) was confirmed in U2-OS cells transfected with HA-Runx2 by immunofluorescence.