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. Author manuscript; available in PMC: 2013 Dec 31.
Published in final edited form as: J Cell Biochem. 2009 Oct 1;108(2):10.1002/jcb.22263. doi: 10.1002/jcb.22263

Figure 2. CoAA Binds the Runt Domain of Runx Factors.

Figure 2

A. This diagram illustrates the GST-Runx2 and GST–Runx1 fusion proteins used in this assay. RuntD is the Runt domain, which is 93% identical in Runx1 and Runx2. B. GST or the indicated GST-Runx fusion proteins were isolated from E. coli lysates with glutathione beads. These beads were incubated with in vitro transcribed and translated, 35S-labeled CoAA. Following extensive washing, the proteins were eluted from the beads with heat, resolved by SDS-PAGE and visualized by autoradiography.