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. Author manuscript; available in PMC: 2013 Dec 31.
Published in final edited form as: J Cell Biochem. 2009 Oct 1;108(2):10.1002/jcb.22263. doi: 10.1002/jcb.22263

Figure 7. CoAA Suppression Blocks Osteosarcoma Cell Growth In Vitro.

Figure 7

A. CoAA is expressed at high levels in human osteoblast-derived cell lines. Cell extracts from the indicated cell lines were resolved by SDS-PAGE and immunoblotted with antibodies to CoAA or LaminB. B. siRNAs decrease CoAA expression. U2-OS cells were incubated for 2 days with control or CoAA siRNAs. Cell extracts from the indicated cell lines were resolved by SDS-PAGE and immunoblotted with antibodies to CoAA or LaminB. C. CoAA suppression reduces U2-OS cell viability. U2-OS cells were transfected with control or CoAA siRNAs for 1, 2, or 3 days. Cell viability was measured with the MTT assay. Results represent the mean of triplicate samples ± SD; **p<0.01.