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The TFS1 multicopy suppressor phenotype of cdc25-1 mutants is dependent on the presence of an intact Ira2 TBD. LRB27 cells expressing a wild-type Ira2p (LRB27), Ira2p lacking its 1,435 C-terminal residues (LRB27Δira2GAP), or Ira2 lacking its 847 C-terminal residues (LRB27Δira2TBD) was transformed or not transformed with the following plasmids: Yep352 and Yep352T. Each was then streaked out for single colonies and grown at 25°C or at the restrictive temperature of 37°C on YPD medium.
