TABLE 2.
Plasmids used in this study
| Plasmid | Description | Source or reference |
|---|---|---|
| Yep352 | 2μm URA3 | 22a |
| Yep352T | Yep352, TFS1 | See text |
| Yep352TP99L | Yep352, TFS1(P99L) | See text |
| Yep352TH111A | Yep352, TFS1(H111A) | See text |
| Yep352TR162A | Yep352, TFS1(R162A) | See text |
| Yep352Y | Yep352, YLR179c | See text |
| pYX243 | 2μm LEU2 | R&D Systems |
| pYXIra2TBD | pYX243, GAL1-IRA2 TBD-HA | See text |
| pYXIra1 | pYX243, GAL1-IRA1 (1987-2358)-HA | See text |
| pKA | kanMX′ | 14 |
| pKAT | pKA, TFS1′ | See text |
| pKAY | pKA, YLR179c′ | See text |
| pAN | ′kanMX | 14 |
| pANT | pAN, ′TFS1 | See text |
| pEGKG | 2μm LEU2-d, URA3, GST-fusion expression vector | 28a |
| pEGKGIra2TBD | pEGKG, IRA2 TBD | See text |
| pGEXIra2-383 | Ampr, plac, GST-Ira2 (1644-2026) | 30 |
| pGEXIra2-704 | GST-Ira2 (1644-2347) | See text |
| pEG202 | 2μm HIS3, LexA DBD, NLS | MoBiTec |
| pEG202T | pEG202, TFS1 | See text |
| pEG202TP99L | pEG202, TFS1(P99L) | See text |
| pEG202TH111A | pEG202, TFS1(H111A) | See text |
| pEG202TR162A | pEG202, TFS1(R162A) | See text |
| pEG202Y | pEG202, YLR179c | See text |
| pEG202Ira2TBD | pEG202, IRA2 TBD | See text |
| pJG4-5 | 2μm TRP1, transcriptional AD | MoBiTec |
| pJG4-5b | pJG4-5, IRA2 (1983-2432) | See text |
| pJG4-5c | pJG4-5, IRA2 (1992-2444) | See text |
| pJG4-5f | pJG4-5, IRA2 (1920-2347) | See text |
| pJGIra2TBD | pJG4-5, IRA2 TBD | See text |
| pJGIra2TBDΔ | pJG4-5, IRA2 TBD Δ 2232-2347 | See text |
| pJGIra1 | pJG4-5, IRA1 (1987-2358) | See text |
| pJGT | pJG4-5, TFS1 | See text |
| pJGY | pJG4-5, YLR179c | See text |
| pGNG | 2μm URA3, LexAop-GFP | MoBiTec |
| pET14-b | Ampr, pT7-His6 | Novagen |
| pET14T | pET14-b, pT7-His6-TFS1 | See text |
| pCR2.1 TOPO | Ampr, Knr, 3′-T, topoI activated | Invitrogen |