TABLE 4.
Two-hybrid pairings tested for GFP fluorescence and Leu2 prototrophy
| Bait | Result for indicated preya
|
||
|---|---|---|---|
| Ira2 TBD (Leu prototrophy and GFP activity) | Ira2TBDΔ (Leu prototrophy and GFP activity) | Ira1 (Leu prototrophy and GFP activity) | |
| Tfs1 | Yes | No | No |
| Tfs1: P99L | No | NDb | ND |
| Tfs1: H111A | No | ND | ND |
| Tfs1: R162A | No | ND | ND |
| YLR179c | No | ND | No |
a
Yes stands for Leu prototrophy and GFP activity in the two-hybrid test. No stands for no Leu prototrophy or GFP activity in the two-hybrid test.
b
ND, not determined.