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Truncated Hap4 can support yeast growth in lactate medium. A yeast strain (JSY4) bearing a chromosomal disruption of HAP4 was transformed with TRP1-marked ARS/CEN plasmids encoding either full-length Hap4(1-554), truncated Hap4(1-330), or no Hap4 protein. Densities of the yeast cultures (measured as OD595) were monitored after a saturated culture was diluted into synthetic medium lacking tryptophan and containing 2% lactate as the sole carbon source.
