FIG. 4.

Transcriptional activation by fragments of the Hap4 C-terminal domain (aa 330 to 554). (A) Schematic diagram depicting Hap4 fragments that were fused to LexA and expressed from the ADH1 promoter on TRP1-marked ARS/CEN plasmids in yeast strain JSY03. (B) β-Galactosidase activities were measured in extracts of three parallel log-phase cultures as described for Fig. 2; means and standard deviations are reported. (C) Immunoblot of lysates from cells expressing various LexA-Hap4 fusion proteins, detected with monoclonal antibodies specific for the LexA DNA-binding domain.